DNA and General PCR Methods: Allele-specific PCR
نویسندگان
چکیده
منابع مشابه
CLN3 by allele specific PCR
The recent isolation of the CLN3 gene involved in Batten disease (juvenile neuronal ceroid lipofuscinosis) creates possibilities for direct detection of mutations which can confirm or indicate the clinical diagnosis of Batten disease. We have designed a rapid and reliable allele specific PCR test for the detection of the major deletion, which can be used in carrier diagnosis, presymptomatic dia...
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Efficient homologous recombination permits the directed introduction of specific mutations into the yeast genome. Here we describe a cloning-free, PCR-based allele replacement method that simplifies allele transfer between yeast strains. The desired allele from one strain is amplified by PCR, along with a selectable/counterselectable marker. After transformation, the resident allele in the targ...
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The detection of single nucleotide polymorphisms (SNPs) is becoming increasingly important in both the evaluation of candidate gene polymorphisms and fine mapping studies (4,7). Many techniques exist for the detection of SNPs within random sequences, such as single-strand conformation polymorphism (SSCP) (5,6), heteroduplex analysis and direct sequencing, but few techniques specifically identif...
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The ease and advantages of allelespecific PCR (AS-PCR) for SNP genotyping, along with the difficulty of assay specificity with certain mismatched DNA primers, have been described in prior studies (1–3). More recently, the use of real-time PCR detection formats has been described for one or both alleles of AS-PCR (4–6). These detection enhancement methods do not overcome the inherent difficultie...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2010
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113361